Directorio de servicios

Directorio de servicios


EPSTEIN BARR / Viral Load

Categoría: Infectious diseases.
Días hábiles de entrega: 7
 

3 ml Venous blood EDTA tube cap Address.

Store and ship samples at temperatures between approximately 4-8 ° C; refrigerant gels or refrigerator ice can be used for it.

DNA extraction from the sample using commercial kits.

Real Time PCR using Epstein Barr Virus Fluorescent Polymerase Chain Reaction Diagnostic Kit DAAN DIAGNOSTICS. For quantification, a standard curve is constructed from the Reference Positive EBV Preparation (1.0x107 UI / ml - 1.0x104 UI /ml) supplied in the kit. The amplification of EBV DNA in the analyzed sample is detected as an increase in fluorescence. The technique is performed in a DA-7600 Thermocycler (DaAn Gene Co.).

Positive control: Positive control supplied by the diagnostic kit.

08 working days.

Negative: DNA amplification is not detected EPSTEIN BARR LOG:--

Positive: DNA detection EPSTEIN BARR Copies / ml, LOG: x

Limit of detection 5000 Copies / ml. It is recommended that absolute values of viral load obtained by genomic amplification procedures be interpreted as any value within a range of 0.5 log (+/- 300 times) of the value obtained.

Recently, it has been proposed that frequent monitoring of EBV DNA loading by real-time PCR (TR-PCR) can be used as an early marker for deciding treatment in cases of active infection, especially when the rapid course is suspected of a VEB-ELP. Some case reports have shown that EBV DNA levels do not reach significant values despite the existence of active disease, so a close assessment should always be made based on the clinical condition.7-9. In addition to the problems of interpretation of the results of PCR-TR studies related to the clinical moment of the patient's infection, add different setbacks that must be faced in the laboratories, regarding the procedures of carrying out the study. There is variability in the results when laboratory tests already commercialized have been evaluated, compared to those carried out locally in each laboratory (homemade), sometimes by some differences in reagents used, denaturation temperature, etc. This requires the laboratories to verify the reproducibility of the tests, with inter-test and intra-test evaluations, with strict controls of the different variables and following the protocols established by the producing laboratories.

Recently, it has been proposed that frequent monitoring of EBV DNA loading by real-time PCR (TR-PCR) can be used as an early marker for deciding treatment in cases of active infection, especially when the rapid course is suspected of a VEB-ELP. Some case reports have shown that EBV DNA levels do not reach significant values despite the existence of active disease, so a close assessment should always be made based on the clinical condition.7-9. In addition to the problems of interpretation of the results of PCR-TR studies related to the clinical moment of the patient's infection, add different setbacks that must be faced in the laboratories, regarding the procedures of carrying out the study. There is variability in the results when laboratory tests already commercialized have been evaluated, compared to those carried out locally in each laboratory (homemade), sometimes by some differences in reagents used, denaturation temperature, etc. This requires the laboratories to verify the reproducibility of the tests, with inter-test and intra-test evaluations, with strict controls of the different variables and following the protocols established by the producing laboratories.

Epstein Barr Fluorescent Polymerase Chain Reaction Diagnostic Kit. http://www.daangene.com.

Watzinger F, Suda M, Preuner S, Baumgartinger R, Ebner K, Baskova L, et al. Real-time quantitative PCR tests for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients. J Clin Microbiol 2004;42:5189-5198.



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